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Susceptibilidad de Cyclocephala comata a diferentes agentes de control biológico

by Posos Ponce, P; Carreón Amaya, J; Serratos Arévalo, J.C; Guerrero Rodríguez, E; Martínez Ramírez, J.L.
Publisher: Abr 2004ISSN: 1659-0082.Subject(s): CYCLOCEPHALA | COLEOPTERA | METARHIZIUM ANISOPLIAE | STEINERNEMA | HETERORHABDITIS | EXTRACTOS VEGETALES | HONGOS ENTOMOPATOGENOS | NEMATODOS ENTOMOFILOS | AGENTES DE CONTROL BIOLOGICO | BIOPLAGUICIDAS | DOSIFICACION | TOXICIDAD | CONTROL BIOLOGICO | CONTROL DE PLAGAS | CYCLOCEPHALA | COLEOPTERA | METARHIZIUM ANISOPLIAE | STEINERNEMA | HETERORHABDITIS | PLANT EXTRACTS | ENTOMOGENOUS FUNGI | ENTOMOPHILIC NEMATODES | BIOLOGICAL CONTROL AGENTS | BIOPESTICIDES | DOSAGE | TOXICITY | BIOLOGICAL CONTROL | PEST CONTROL | CYCLOCEPHALA | COLEOPTERA | METARHIZIUM ANISOPLIAE | STEINERNEMA | HETERORHABDITIS | EXTRAIT D'ORIGINE VEGETALE | CHAMPIGNON ENTOMOPATHOGENE | NEMATODE ENTOMOPATHOGENE | AGENT DE LUTTE BIOLOGIQUE | BIOPESTICIDE | DOSE | TOXICITE | LUTTE BIOLOGIQUE | LUTTE ANTIRAVAGEUR | CYCLOCEPHALA COMATAOnline Resources: Es In: Manejo Integrado de Plagas y Agroecología (CATIE) (no.71) p. 92-95Summary: El objetivo de esta investigación fue determinar la dosis letal media (DL50) de extractos de plantas (ajo, cebolla y nim) y algunos agentes entomopatógenos. Los productos fueron diluidos en agua destilada y posteriormente se procedió a correr los bioensayos de cada bioinsecticida sobre 20 larvas. Los tratamientos a base de ajo, cebolla y nim no mostraron efecto de control sobre las larvas de C. comata. La población se mostró sensible al hongo entomopatógeno Metarhizium anisopliae, obteniendo la DL50 con 2.1 x 10 exponente 11 conidios por larva. Con el namtodo Steinernema, la DL50 se obtuvo con 209 nematodos/larva, y con Heterorhabditis la DL50 se obtuvo con 42 nematodos/larva.Summary: The objective of our research was to determine the DL50 of plant extracts such as garlic, onion and neem, as well as entomopathogenic agents such as fungi (Metarhizium anisopliae) and nematodes (Steinernema and Heterorhabditis). The products were dissolved in distilled water and a bioassay was carried out for each biopesticide through topical application. For each biopesticide, 9-12 dosage levels were evaluated on 20 larvae. Mortality was corrected by Abbot. results were analyzed by Profit analisis for dosage response. Results were plotted on a logarithmic scale to obtain a slope. Garlic, onion and neem treatments did nt have a controlling effect on larvae. C. comata was very susceptible to the pathogenic fungi M. anisopliae, with a DL50 of 2.1 x 10 exponent 11 conidia/larvae. For the Steinernema nematode, the DL50 was 209 nematodes/larvae, and of 42 nematodes/larvae for Heterorhabditis.
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El objetivo de esta investigación fue determinar la dosis letal media (DL50) de extractos de plantas (ajo, cebolla y nim) y algunos agentes entomopatógenos. Los productos fueron diluidos en agua destilada y posteriormente se procedió a correr los bioensayos de cada bioinsecticida sobre 20 larvas. Los tratamientos a base de ajo, cebolla y nim no mostraron efecto de control sobre las larvas de C. comata. La población se mostró sensible al hongo entomopatógeno Metarhizium anisopliae, obteniendo la DL50 con 2.1 x 10 exponente 11 conidios por larva. Con el namtodo Steinernema, la DL50 se obtuvo con 209 nematodos/larva, y con Heterorhabditis la DL50 se obtuvo con 42 nematodos/larva.

The objective of our research was to determine the DL50 of plant extracts such as garlic, onion and neem, as well as entomopathogenic agents such as fungi (Metarhizium anisopliae) and nematodes (Steinernema and Heterorhabditis). The products were dissolved in distilled water and a bioassay was carried out for each biopesticide through topical application. For each biopesticide, 9-12 dosage levels were evaluated on 20 larvae. Mortality was corrected by Abbot. results were analyzed by Profit analisis for dosage response. Results were plotted on a logarithmic scale to obtain a slope. Garlic, onion and neem treatments did nt have a controlling effect on larvae. C. comata was very susceptible to the pathogenic fungi M. anisopliae, with a DL50 of 2.1 x 10 exponent 11 conidia/larvae. For the Steinernema nematode, the DL50 was 209 nematodes/larvae, and of 42 nematodes/larvae for Heterorhabditis.

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