Normal view MARC view ISBD view

Memoria

by Pereira, A; Bertrand, B; Vásquez, N; Barry-Etienne, D; Etienne, H; Solano, W; Echeverri, J; Zamora, L. comps; IICA, San José (Costa Rica). PROMECAFE; Instituto del Café de Costa Rica, San José (Costa Rica); 19. Simposio Latinoamericano de Caficultura San José (Costa Rica) 2-6 Oct 2000.
Publisher: San José (Costa Rica) 2000Description: p. 273-283; 514 p.ISBN: 9977550247.Other Title: A solution for the utilization of In vitro culture for mass-diffusion of Coffea arabica elite materials: the direct sowing of somatic embryos produced in a bioreactor.Subject(s): COFFEA ARABICA | EMBRIOGENESIS SOMATICA | CULTIVO IN VITRO | SIEMBRA DIRECTA | GERMINACION | COFFEA ARABICA | SOMATIC EMBRYOGENESIS | IN VITRO CULTURE | DIRECT SOWING | GERMINATION | COFFEA ARABICA | EMBRYOGENESE SOMATIQUE | CULTURE IN VITRO | SEMIS DIRECT | GERMINATIONSummary: The effect of germination conditions on the morphology of Coffea arabica L. somatic embryos mass-produced in a one-litre temporary immersion bioreactor (RITA7) was studied with emphasis on direct sowing in soil. Using germinated embryos, direct sowing resulted in a highly successful conversion of embryos into plants. Culture density above 1,600 embryos/11-bioreactor positively affected embryo morphology by causing higher embryonic axis elongation (+4-5mm). At this density, addition of a high sucrose concentration (234 mM), 2 weeks before sowing, promoted an increase in effective plant conversion in soil (78 percent) and a vigorous vegetative growth of the resulting plants. Using this somatic embryogenesis process, 50 000 selected F1 hybrids were recently difunded in America Central.
    average rating: 0.0 (0 votes)
No physical items for this record

Ilus. 2 tab. 22 ref.

The effect of germination conditions on the morphology of Coffea arabica L. somatic embryos mass-produced in a one-litre temporary immersion bioreactor (RITA7) was studied with emphasis on direct sowing in soil. Using germinated embryos, direct sowing resulted in a highly successful conversion of embryos into plants. Culture density above 1,600 embryos/11-bioreactor positively affected embryo morphology by causing higher embryonic axis elongation (+4-5mm). At this density, addition of a high sucrose concentration (234 mM), 2 weeks before sowing, promoted an increase in effective plant conversion in soil (78 percent) and a vigorous vegetative growth of the resulting plants. Using this somatic embryogenesis process, 50 000 selected F1 hybrids were recently difunded in America Central.

Click on an image to view it in the image viewer