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Anther and endosperm culture of coffee

by Raghuramulu, Y.
Publisher: Jul 1989Subject(s): COFFEA | CULTIVO DE ANTERAS | INOCULACION | MICROSPOROGENESIS | CULTIVO DE TEJIDOS | CALLO | ENDOSPERMA | INDIA | COFFEA | ANTHER CULTURE | INOCULATION | TISSUE CULTURE | CALLUS | ENDOSPERM | INDIA | COFFEA | CULTURE D'ANTHERE | INOCULATION | CULTURE DE TISSU | CAL | ENDOSPERME | INDE In: Journal of Coffee Research (India) v. 19(2) p. 71-81Summary: Anthers inoculated at microsporogenesis stage (i.e., three days from the receipt of blossom showers) produced profuse callus on a modified M.S. medium supplemented with 0.2 mg/l each of IAA and BAP. However, these calluses failed to respond to further subcultures. On the otherhand, immature endosperm collected from six-month old fruits of robusta coffee produced callus and subsequently turned embryonic on subculturing
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Anthers inoculated at microsporogenesis stage (i.e., three days from the receipt of blossom showers) produced profuse callus on a modified M.S. medium supplemented with 0.2 mg/l each of IAA and BAP. However, these calluses failed to respond to further subcultures. On the otherhand, immature endosperm collected from six-month old fruits of robusta coffee produced callus and subsequently turned embryonic on subculturing

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