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Caffeine as main interfering compound in radioimmunoassay of aflatoxin B1 in coffee samples

by Rauch, P; Víden, I; Davidek, T; Velisek, J; Fukal, L.
Publisher: Nov-Dic 1989Subject(s): CAFE | CAFEINA | EXTRACTOS | CROMATOGRAFIA DE GASES | ANALISIS QUIMICO | AFLATOXINAS | GRANOS | CAFE DESCAFEINIZADO | COFFEE | CAFFEINE | EXTRACTS | GAS CHROMATOGRAPHY | AFLATOXINS | GRAIN | CAFE | CAFEINE | EXTRAIT | CHROMATOGRAPHIE EN PHASE GAZEUSE | AFLATOXINE | GRAIN In: Journal of the Association of Official Analytical Chemists (EUA) v. 72(6) p. 1015-1017Summary: The content of caffeine in coffee extracts prepared for radioimmunoassay of aflatoxin B1 was determined by gas chromatography. The extracts from coffee beans and decaffeinated coffee contained 1.76-4.60 and 0.71-0.85 g caffeine/kg, respectively. These concentrations of caffeine caused false results in radioimmunoassay of aflatoxin B1 in the range 1.0-2.8 ug/kg for coffee beans and 0.3-0.4 ug/kg for decaffeinated coffee
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The content of caffeine in coffee extracts prepared for radioimmunoassay of aflatoxin B1 was determined by gas chromatography. The extracts from coffee beans and decaffeinated coffee contained 1.76-4.60 and 0.71-0.85 g caffeine/kg, respectively. These concentrations of caffeine caused false results in radioimmunoassay of aflatoxin B1 in the range 1.0-2.8 ug/kg for coffee beans and 0.3-0.4 ug/kg for decaffeinated coffee

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