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Clonal propagation of sugar cane Biotechnology in the Americas 2: applications in tropical agriculture, a symposium

by Lee, T.S.G. Instituto do Acucar e Alcool, Sao Paulo, SP (Brasil); Mora Poltronieri, H. ed; Interciencia Association, Washington, D.C. (EUA); Consejo Nacional de Investigaciones Científicas y Tecnológicas, San José (Costa Rica); Symposium Biotechnology in the Americas. Applications in Tropical Agriculture San José (Costa Rica) 14-17 Jul 1985.
Publisher: San José (Costa Rica) 1985Description: p. 58.Subject(s): CULTIVO DE TEJIDOS | CANA DE AZUCAR | CLONES | TISSUE CULTURE | SUGARCANE | CLONES | CULTURE DE TISSU | CANNE A SUCRE | CLONESummary: Tissue culture can be used to solve these problems. The technique has been applied intensely for many horticultural plants. However, few investigations have been carried out on sugar cane. Since 1981, research with sugar cane tissue culture has been carried out by the Physiology Section of PLANALSUCAR - Institute of Sugar and Alcohol (IAA). One of the subjects under study is clonal propagation of sugar cane. Sugar cane meristem tips (about 2 mm in size) have been cultured into shoots and then multiplied in a cytokinin-containing-liquid-MS medium without shaking. Starting from a single meristem tip, it is possible to produce 100,000 to 400,000 plantlets or more, within 6 months, depending on the variety. Rooting was obtained by transplanting the shoots directly into vermiculite in a greenhouse at 24-30 grades C and a relative humidity of 70-90 per cent. Roots usually formed after 1 week and the survival rate was higher than 80 per cent. Plants produced by this method were similar to the mother plant although more vigor was noted with the meristem plants. Comparisons of productivity of sugar cane using material of both meristem plants and mother plants is now being carried out
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Tissue culture can be used to solve these problems. The technique has been applied intensely for many horticultural plants. However, few investigations have been carried out on sugar cane. Since 1981, research with sugar cane tissue culture has been carried out by the Physiology Section of PLANALSUCAR - Institute of Sugar and Alcohol (IAA). One of the subjects under study is clonal propagation of sugar cane. Sugar cane meristem tips (about 2 mm in size) have been cultured into shoots and then multiplied in a cytokinin-containing-liquid-MS medium without shaking. Starting from a single meristem tip, it is possible to produce 100,000 to 400,000 plantlets or more, within 6 months, depending on the variety. Rooting was obtained by transplanting the shoots directly into vermiculite in a greenhouse at 24-30 grades C and a relative humidity of 70-90 per cent. Roots usually formed after 1 week and the survival rate was higher than 80 per cent. Plants produced by this method were similar to the mother plant although more vigor was noted with the meristem plants. Comparisons of productivity of sugar cane using material of both meristem plants and mother plants is now being carried out

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